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Our main code repository for analyzing data collection from UCE loci.Contains command-line applications for assembling contigs from sequence data, finding which contigs align to UCEs, aligning UCE contigs, and preparing data for downstream analysis in mrbayes, raxml, and cloudforest. Get phyluce » A program for demultiplexing massively parallel sequencing reads tagged with edit distance or Hamming distance sequence tags - tailored to edit distance tags (see Tags).Get 2,001 probe set for fish » Below are several software packages we have developed to help analyze data collected from UCE loci.All computer code is available under a flexible open-source license (BSD).We welcome all code contributions, from helping to improve the code, fix bugs, improve usability, and improve documentation, which is rather sparse, at the moment.Please contact us through twitter (@ultraconserved) if you are interested in helping and/or post an issue on github for the respective package.Get protocols » The most complex part of using UCEs to understand evolutionary relationships, population structure, and population relationships is analyzing the DNA sequence data. Mc Cormack JE, Harvey MG, Faircloth BC, Crawford NG, Glenn TC, Brumfield RT. A Phylogeny of Birds Based on Over 1,500 Loci Collected by Target Enrichment and High-Throughput Sequencing. Generally speaking, these commercial vendors will accept DNA extracts for enrichment, conduct the library preparation and enrichment steps, sequence the enriched libraries, and return the sequence data to you. We are constantly evaluating the utility of given probe sets and probe designs, in addition to expanding the number of UCE loci we are targeting.We have created several software packages and we're working on tutorials to help get you started. A Phylogenomic Perspective on the Radiation of Ray-Finned Fishes Based upon Targeted Sequencing of Ultraconserved Elements (UCEs). We do not derive any referall revenue from these companies, but we list each here to help interested labs get started. We have several larger probes sets in the works, and we are also working on optimizing probe sets based on their capture success, phylogenetic utility, etc. You can now buy each of these probe sets direct from MYcroarray in the form of a capture kit.
Because of the highly conserved nature of UCEs and their flanking sequence, we have found these probes work well across fishes.Many of the steps, at this point, require that you are comfortable working with computer software on the command line. MYcroarray has even made a discounted "pilot" sized kit available for labs who want to do some test enrichments.We encourage everyone interested to get the software and contribute to the effort of documenting, improving, and extending our computer code. Order enrichment kits from MYcroarray » We designed probes from UCEs by including flanking sequence from chickens.Capable of demultiplexing hundreds of sequence tagged libraries at once. Get splitaake » A program for automated cleaning of fastq files from sequencing.Removes adapter contamination using scythe and trims reads for quality using sickle.